Bioengineering/Scientific Research III

SCBE: Bioengineering/Scientific Research III

SCBE.A: Content

SCBE.A.1: obtain, evaluate, and communicate information on employing the use of Standard Laboratory Operating Procedures (SLOP) throughout the course

SCBE.A.2: obtain, evaluate, and communicate information regarding the bioengineering field and its application in society

SCBE.A.3: obtain, evaluate, and communicate information focused on the ethical and legal issues arising from the application of bioengineering

SCBE.A.4: obtain, evaluate, and communicate information about career opportunities in the field of bioscience

SCBE.A.5: obtain, evaluate, and communicate information about how basic chemistry concepts affect living organisms

SCBE.A.6: obtain, evaluate, and communicate information on applying technologies used in the life science industry

SCBE.A.7: obtain, evaluate, and communicate information about the development and delivery of biotechnology and bioengineering to the marketplace

SCBE.A.1.a: set up and maintain a legal scientific notebook in order to communicate information

SCBE.A.1.b: measure volume accurately using graduated cylinders, pipets, and micropipettes

SCBE.A.1.c: measure mass accurately using electronic balances

SCBE.A.1.d: use computational thinking to calculate and prepare solutions based on mass/volume, % mass/volume, and molar concentration

SCBE.A.1.e: prepare solutions of calculated volume and concentration

SCBE.A.1.f: prepare solutions from stock concentrated solutions

SCBE.A.1.g: calculate and perform serial dilutions

SCBE.A.1.h: plan and carry out investigations demonstrating correct skills and use of common lab equipment

SCBE.A.1.i: use models to explain the steps in cell culture, sterile technique, and media preparations

SCBE.A.1.j: plan and carry out investigations investigations using correct microscope use and slide preparation techniques, including simple and Gram staining

SCBE.A.1.k: record measurements and calculations adhering to significant figure rules

SCBE.A.1.l: use models to implement SDS and NFPA information to ensure workplace safety

SCBE.A.1.m: analyze, interpret data, and identify and/or calculate experimental error

SCBE.A.1.n: engaging in argument from evidence regarding the importance of accuracy and precision during investigations

SCBE.A.2.a: define terms commonly used in the biotechnology and bioengineering field

SCBE.A.2.b: use models to identify and use applications of bioengineering in society

SCBE.A.2.c: engaging in argument from evidence regarding the history and use of model organisms in bioengineering

SCBE.A.3.a: engage in arguments related to the ethical concerns focused on genetically modified foods, cloning, gene editing, bioterrorism, gene therapy, and stem cells

SCBE.A.3.b: analyze and discuss the ethical and legal issues related to bioengineering

SCBE.A.3.c: ask questions and analyze possible contamination factors and how they may impact bioengineering protocols

SCBE.A.4.a: explore bioengineering career diversity

SCBE.A.4.b: support arguments for pursuing careers in biotechnology and bioengineering at differing entry levels

SCBE.A.4.c: develop and use models to demonstrate literacy and performance based skills used in the biotechnology and bioengineering field

SCBE.A.5.a: compare and contrast cellular design and function in plant, animal, and bacterial cells

SCBE.A.5.b: develop and use models to promote cell growth under aseptic conditions

SCBE.A.5.c: plan and carry out investigations monitoring the growth of cell cultures

SCBE.A.5.d: use models to describe the structure and function of the four organic macromolecules that comprise cells including carbohydrates, lipids, proteins, and nucleic acids

SCBE.A.5.e: plan and carry out investigations with macromolecules using indicator tests including Biuret, Benedict's, and Lugols

SCBE.A.5.f: use the central dogma of biological sciences to model and communicate the role of DNA in organisms (DNA-> RNA-> protein-> function)

SCBE.A.5.g: compare and contrast cell growth and reproduction, DNA replication, mitosis and meiosis, and protein synthesis

SCBE.A.5.h: use models to analyze nucleic acid structure and function

SCBE.A.5.i: carry out investigations in order to extract genomic DNA from cells

SCBE.A.5.j: carry out investigations in order to prepare, load, run, and visualize DNA samples on agarose gel

SCBE.A.5.k: carry out Polymerase Chain Reaction (PCR) to manipulate DNA

SCBE.A.5.l: engage in arguments and provide examples of the importance of genetic variation in a gene pool

SCBE.A.5.m: use models to analyze the relationship between protein structure and function

SCBE.A.5.n: carry out investigations to analyze protein concentration in solution

SCBE.A.5.o: carry out investigations in order to prepare, load, run, and visualize protein samples on polyacrylamide gel

SCBE.A.5.p: plan and carry out investigations exploring the use of enzymes in bioengineering

SCBE.A.5.q: analyze and interpret enzyme activity assays

SCBE.A.6.a: use models to describe the properties of buffers and solutions

SCBE.A.6.b: use pH paper and meter models to measure and adjust the pH of a solution

SCBE.A.6.c: analyze how pH affects protein structure and function

SCBE.A.6.d: use models to show how assays for reactants and products can be used to indicate the presence or activity of an enzyme

SCBE.A.6.e: construct explanations of the role ELISA assays play in industry

SCBE.A.6.f: plan and carry out investigations to conduct an ELISA assay testing the presence of a protein

SCBE.A.6.g: use models to show the use and applications of a spectrophotometer

SCBE.A.6.h: use models to describe genetic manipulation and the use of recombinant DNA

SCBE.A.6.i: plan and carry out investigations conducting bacterial transformation

SCBE.A.6.j: use models to argue the research application of genetically modified organisms

SCBE.A.6.k: explore the use of antigens and antibodies in immunological biotechnology

SCBE.A.6.l: use models and computational thinking to run guided queries, such as BLAST, as an introduction to bioinformatics

SCBE.A.6.m: use models and databases to analyze genomic and proteomic query results

SCBE.A.7.a: use models to review current trends in the bioengineering industry

SCBE.A.7.b: develop and use models used during bioengineering product development (product pipeline)

SCBE.A.7.c: identify several products created using recombinant DNA technology

SCBE.A.7.d: use models to describe the use of plant extracts in the pharmaceutical industry

SCBE.A.7.e: plan and carry out investigations testing plant extracts for anti- microbial activity

SCBE.A.7.f: use models to examine and argue the role of regulatory agencies and processes used in bioengineering